The 22-year-old has suffered fertility problems this season.
Sam Bullard, Darley's Director of Stallions, said: "We are approaching the end of March and he has failed to get a mare in foal, so it has been decided that he should be retired from stallion duties.
"Breeders have been kept informed throughout," he added.
Cape Cross retired to stud in 2000 after ending his racing days with a fine record of five wins from 19 starts, which included victory in Newbury's G1 Lockinge Stakes.
Apart from Golden Horn, he sired Champion Sea The Stars and the multiple Classic winner Ouija Board.
